Device

Part:BBa_K1913006:Design

Designed by: Thomas Mathijs Swartjes   Group: iGEM16_Wageningen_UR   (2016-09-30)


434- and lambda cI balance operon + mRFP reporter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1898
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
    Illegal AgeI site found at 4249
    Illegal AgeI site found at 4361
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961


Design Notes

I chose to digest the mRFP operon with SpeI and PstI and ligate it into pSB1C3 with the Arabinose inducbile operon digested with XbaI and PstI. This would (and did) result in a plasmid that contains both operons in the most understandable order and colours successful clones light-red.


Source

The part has been assembled from parts BBa_K1913007 and BBa_K1913016 via digestion a subsequent ligation. BBa_K1913007 and BBa_K1913016 were both assembled through Gibson assembly from fragments derived from part in the iGEM registry.

References